AEGIS Potato project
The identification of 500 old potato clones having
unreliable variety name by means of fingerprinting using 12 microsatellite
(SSR) markers to assist in setting up the AEGIS collection for potato cultivars.
What's new ...
- May 1, 2017: Contact information updated in Excel file AegisPotato_selection
- October 9, 2014: Technical report of the project finalized.
- September 9, 2014: Minimum list of 17 genotypes with all 95 alleles in this study incuded in the Excel file. Minor improvement in VBA subroutine SetIniValues.
- September 5, 2014: Table with allele sizes from a 3500xl sequencer (at SASA) included in the Excel
- August 15, 2014: Updated report (but still not the final version)
- July 25-11, 2014: Data export macro's for NTSys and Darwin included in the Excel data file
- July 7-11, 2014: Poster presentation at the 19th EAPR triennial conference in Brussels
- December 20, 2013: Genotyping results on 30 Irish clones included in the overall data set (by SASA)
- November 18, 2013: SSR results on a selection of 243 clones from Ireland announced to become available to Aegis
- November 1, 2013: SSR genotyping results available on 403 samples, incl. 135 heritage varieties from the UK.
- March 6, 2013: 34 samples shipped by NORDGEN, Sweden
- February 28, 2013: 28 samples shipped by INRA, France
- February 2, 2013: so far SASA received 136 samples from Germany, 4 from Ukraine (requested by Estonia), 25 from Switzerland, 39 from the Czech Republic and 2 from Latvia
- December 5, 2012: 28 clones requested from France
- December 3, 2012: notification from Ireland: the collection was genotyped by SASA 3 years ago, identifying 25% duplicates among old varieties
- November 16, 2012: draft list of 100 varieties available from France, that might be put into the MLS
- July, 2012: selection finalised of 441 accessions (based on Eurisco), leaving room for 59 other clones
- December 21, 2011: budget neutral extension of the project till end 2012
- June 20, 2011: agreement/contract signed by CGN and AEGIS.
The original available AEGIS project budget of 10k was raised to 26,8k euro by ECPGR, by including the budget available for the ECPGR Potato Working Group.
- December 31, 2010: project proposal 'Identification of old potato clones by means of microsatellites' submitted to the Competitive AEGIS Grant Scheme by CGN.
Selection criteria for choosing material for the fingerprinting
- only material that has been put into the Multi-Lateral-System (MLS) by the collection holder,
therefore unfortunately excluding collections from France, Spain & Italy.
- UK collection skipped under the assumption that it is already completely genotyped by SASA
- only old varieties <=1960, or more modern varieties with the same name as an elder clone (e.g. Apollo).
- samples from country of origin prefered over other collections.
- preferably heritage varieties that have not been profiled by SASA before to enshrine the fingerprints
Feedback to: Roel.Hoekstra@wur.nl,
Centre for Genetic Resources the Netherlands (CGN), Wageningen, the Netherlands.