EU project RESGEN CT99 109-112
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EU project RESGEN CT99 109-112
"Brassica Collections for Broadening Agricultural Use" including "Characterising and utilising genetic variation in Brassica carinata for its exploitation as an oilseed crop"
The RESGEN CT99 109-112 project on the genetic resources of Brassica was carried out in the framework of the European Community Programme on the Conservation, Characterisation, Collection and Utilisation of Genetic Resources in Agriculture. The project was funded by The European Commission and started January 1st 2000.
The project is a fusion of two separate proposals RESGEN 109 "Brassica collections for broadening agricultural use" and RESGEN 112 "Characterising and utilising genetic variation in Brassica carinata for its exploitation as an oilseed crop"
The project aims to conserve, document, characterise, evaluate and rationalise European collections of four important Brassica species. this will contribute to a better knowledge of the genetic resources of these important Brassica species and improve the utilisation of the genepools in Europe by plant breeders and growers. The project deals with the following Brassica species:
European database for Brassica (Bras-EDB)
The Bras-EDB was updated twice during the four-year project period. This database focuses on passport data of collections of the Genus Brassica maintained in European countries. The version 2001 of the Bras-EDB included 32 collections of 22 countries. This version contains 19110 accessions of both cultivated and wild material.
The version 2004 was extended with a few new collections and further updated and improved with addresses in line with the FAO/IPGRI Multi-crop Passport Descriptors. The database contains passport data of c.19.600 accessions and includes 36 collections of 22 countries. The version 2004 of the Bras-EDB will be made on line searchable on the Internet in 2005. In this version the accessions in the core collections from EU AIR Project (1993-1997) and this RESGEN project have been tagged in a seperate field and are also part of the search criteria.
Core collections of the four Brassica species
Each of the four subgroups of the project developed their own core collection. The size of the four core collections is as follows:
Characterisation and regeneration
The subgroups agreed upon sets of minimum descriptors for each of the four Brassica species. The cores and parts of the collections were characterised for these descriptors. In total the project characterised over 1250 accessions. A large part of the B. carinata collection was characterised using DNA finger printing.
The project regenerated some 889 accessions divided over all four Brassica species.
Evaluation
More than 4800 samples were evaluated for a range of properties (see overview). Results of these evaluation experiments will be linked to the Bras-EDB. The different evaluations resulted in the detection of accessions with interesting properties for further breeding. See also the "fourth year and final report".
Overview of the evaluations conducted
| Property | Number of properties | Brassica species |
| Pest and diseases | 11 | All four Brassica spp. |
| Quality | 4 | B. napus, B. rapa, B. carinata |
| Salt Tolerance | 1 | B. oleracea |
| Agronomic evaluation | 2 | B. carinata |
Safety duplication
The system of safety duplication of Brassica collections in Europe has been highly improved compared to the situation at the beginning of the project in 2000. The majority of the genebanks have now safety duplicated their Brassica collections in another place.
Gaps in the collections
All subgroups identified gaps in the respective four Brassica species. This information can be seen as a guideline for the future to fill the gaps in the existing Brassica collections.
Dissemination of information
The results of the project were disseminated by means of the "fourth year and final report", a workshop, publications and presentations and the Internet (Bras- EDB is on line searchable)
The following data can be downloaded in Zipped Excel format.
Astley, D. & A. Pinnegar, 2003. Progress of the Brassica rapa group in the GENRES CT99 109-112 Project. Pp. 59-62. In: Maggioni, L., G. Thomas and E. Lipman, compilers. Report of a Working Group on Brassica. Extraordinary Meeting, held jointly with the Third Coordination Meeting of the EU project GENRES 109-112, 8-9 February 2002, Vila Real, Portugal. IPGRI, Rome, Italy.
Angela Pinnegar, Dave Astley, Klaus Dehmer, Marie-Luise Graichen, Evelin Willner, Eduardo Rosa, Alfredo Aires, Rosa Paula, Amando Ordas & Elena Cartea, 2004. Brassica Collections for Broadening Agricultural Use: RESGEN CT99 109/112, Brassica rapa Subgroup. Cruciferae Newsletter nr.25, 113-114
Bas, N., I. W. Boukema & L. J. M. van Soest, 2003. Role and progress of the Brassica oleracea group in the GENRES CT99 109-112 project after 2 years. Pp. 55-58. In: Maggioni, L. and G. Thomas and E. Lipman, compilers. Report of a Working Group on Brassica. Extraordinary Meeting, held jointly with the Third Coordination Meeting of the EU project GENRES 109-112, 8-9 February 2002, Vila Real, Portugal. IPGRI, Rome, Italy.
Boukema, I.W., N. Cristea, Th.J.L van Hintum & F. Menting, 2003. The European Brassica Database: version 2001.Pp. 15-18. In: Maggioni, L. and G. Thomas, compilers. Report of a Working Group on Brassica. Extraordinary Meeting, held jointly with the Third Coordination Meeting of the EU project GENRES 109-112, 8-9 February 2002, Vila Real, Portugal. IPGRI, Rome, Italy.
Ietje W. Boukema, Noor Bas, Gregoire Thomas, Nikolaos Stavropoulos, Ferdinando Branca, Maria Jose Diez and Kees van ‘t Hoenderdal (representing Plantum NL), 2004. Evaluation, characterisation and regeneration of Brassica oleracea germplasm collections in the EU GENRES CT99 109-112 project ‘Brassica, including B. carinata’. Cruciferae Newsletter nr.25, 107-108
Branca F., 2002. III Meeting del progetto europeo RESGEN CT99 – 109/112 sulle Brassicaceae organizzato a Catania. Notiziario Risorse Genetiche Vegetali, III, 2, 1-3. Istituto Sperimentale per la Frutticoltura, Roma.
Chinoy, C., E. Arthur, I. Bancroft, A. De Haro-Bailón, C. Morgen, 2002. Genetic Variation in Brassica carinata Assessed using Microsatellite Markers and Phenotypic Characters.: 13 th Cruficer Genetics Workshop. Davis, California, 23-26th March 2002
Font, R., M. del Rio, J. Fernández, E. Arthur, I. Bancroft, C. Chinoy, C. Morgan and A. De Haro, 2002 Seed Oil Content Analysis of Ethiopian mustard (Brassica carinata A. Braun) by Near Infrared Spectroscopy. Eucarpia Cruciferae Newsletter. 24: 5-6
Font, R., M. del Rio, A. Sillero, E. Arthur, I. Bancroft, C. Chinoy, C. Morgan and A. De Haro, 2002 Using Near Infrared Spectroscopy for Determining Protein content in Ethiopian mustard (Brassica carinata A. Braun). Eucarpia Cruciferae Newsletter. 24: 7-8
Font, R., Del Río, M., Arthur, E., Bancroft, I., Chinoy, C., Morgan, C., De Haro, A. 2004.Survey of seed storage components in Ethiopian mustard (Brassica carinata A.Braun). Cruciferae Newsletter nr.25, 109-110
Haro de, A., R. Font, J. Navarrete, J. M. Ferandez, E. Arthur, C. Chinoy, I Bancroft and C. Morgan, 2003. Role and progress of the Brassica carinata group in the GENRES CT99 109-112 project after 2 years. Pp. 72-74. In: Maggioni, L. and G. Thomas, compilers. Report of a Working Group on Brassica. Extraordinary Meeting, held jointly with the Third Coordination Meeting of the EU project GENRES 109-112, 8-9 February 2002, Vila Real, Portugal. IPGRI, Rome, Italy.
Hasan M., W. Lühs, F. Seyis, W. Friedt and R. Snowdon, 2003: Surveying genetic diversity in the Brassica napus gene pool using SSR markers. 11th. Intern. Rapeseed Congr, 6-10 July 2003,Copenhagen, Denmark, Vol. 2, pp. 392-394.
Lühs, W., F. Seyis, M. Frauen, H. Busch, L. Frese, E. Willner, W. Friedt, M. Gustafsson, and G. Poulsen, 2002a: Development and evaluation of a European Brassica napus core collection. In: P.B.E. McVetty, K. Schlosser, and C.F. Quiros (Eds.), 13th Crucifer Genetics Workshop, 23-26 March 2002, University of California, Davis, California, USA, Poster abstract, p. 116.
Lühs, W., F. Seyis, M. Frauen, H. Busch, L. Frese, E. Willner, W. Friedt, M. Gustafsson, and G. Poulsen, 2003a: Development and evaluation of a Brassica napus core collection. In: H. Knüpffer & J. Ochsmann (eds.), Rudolf Mansfeld and Plant Genetic Resources. Proceedings of a symposium dedicated to the 100th birthday of Rudolf Mansfeld, Gatersleben, Germany,8-9 October 2001. Schriften zu Genetischen Ressourcen 19, pp. 287-292. ZADI/IBV, Bonn.
Lühs W, B. Ulber, F. Seyis, M. Frauen, H. Busch, W. Friedt, M. Gustafsson and G. Poulsen, 2003b: Evaluation of a Brassica napus core collection and prospects of its exploitation through rapeseed breeding. 11th Intern. Rapeseed Congr., 6-10 July 2003, Copenhagen, Denmark, Vol. 2, pp. 371-374.
Lühs, W., F. Seyis, M. Frauen, H. Busch, L. Frese, E. Willner, W. Friedt, M. Gustafsson, G. Poulsen ,2003c. Preliminary Field Evaluation of a Brassica napus Core Collection. Pp. 67-71. In: Maggioni, L. and G. Thomas and E. Lipman, compilers. Report of a Working Group on Brassica. Extraordinary Meeting, held jointly with the Third Coordination Meeting of the EU project GENRES 109-112, 8-9 February 2002, Vila Real, Portugal. IPGRI, Rome, Italy.
Poulsen G, H. Busch, M. Frauen, L. Frese, M. Gustafsson, F. Seyis, E. Willner, W. Lühs, 2003.Development and Evaluation of a Brassica napus L. Core Collection. Pp. 63-66. Report of a Working Group on Brassica. Extraordinary meeting, held jointly with the Third Coordination Meeting of the EU project GENRES 109-112, 8-9 February 2002 Vila Real, Portugal. IPGRI, Rome, Italy.
Poulsen G., H. Busch, M. Frauen, L. Frese, W. Friedt, M. Gustafsson, F. Ottosson, F. Seyis, G. Stemann, B. Ulber, E. Willner, W. Lühs.(2004) The European Brassica napus Core Collection – Characterisation, Evaluation and Establishment. Cruciferae Newsletter nr.25, 115-116
Seyis, F., R. Snowdon, W. Friedt, W. Lühs, 2003a: Molecular genetic diversity in resynthesisedand conventional spring rapeseed. 11th Intern. Rapeseed Congr., 6-10 July 2003, Copenhagen, Denmark, Vol. 2, pp. 382-384.
Seyis F., R.J. Snowdon, W. Lühs and W. Friedt, 2003b: Molecular characterisation of novel resynthesised rapeseed (Brassica napus L.) lines and analysis of their genetic diversity in comparison to spring rapeseed cultivars. Plant Breeding (in press).
Soest van, L. J. M., I. W. Boukema & N. Bas, 2003. The EU GENRES CT99 109-112 project Brassica, including B. carinata. Pp. 50-54. In: Maggioni, L. and G. Thomas and E. Lipman, compilers. Report of a Working Group on Brassica. Extraordinary Meeting, held jointly with the Third Co-ordination Meeting of the EU project GENRES 109-112, 8-9 February 2002, Vila Real, Portugal. IPGRI, Rome, Italy.
Soest van, L. J. M., I. W. Boukema & N. Bas; The achievements of the EU GENRES CT99 109-112 project Brassica, including B. carinata. Cruciferae Newsletter nr.25, 117-118.
This project has been carried out with financial support from the Commission of the European Communities, Agriculture and Fisheries. It does not necessarily reflect its view and in no way anticipates the Commission's future policy in this area.
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